Effectiveness of omega-3 on some physiological aspects in aged rats’ kidneys
DOI:
https://doi.org/10.30539/3hkaky18Keywords:
Aging, D-gal, kidney functions, oxidative biomarker, omega-3Abstract
Background: The kidneys are particularly susceptible to the effects of aging. A major disruption of the body's homeostasis may occur from unfortunate events. It has been suggested that omega-3 can help reduce the effects of aging caused by D-galactose (D-gal). This study aimed to investigate the potential benefits of omega-3 in reducing dysregulation in kidney functions, redox homeostasis, and inflammatory markers in D-gal- treated rats. Methodology: Sixty adult male rats were randomly divided into four groups as follows: Control group (T1) were injected with normal saline(i/p) and given orally soya bean oil for 90 days, group T2 were injected with D-galactose at 150 mg/kg BW i/p and given omega-3 (75 mg/ kg BW) for 90 days while group T3A were injected with same dose of D-gal for 60 days then given orally omega-3 (75 mg/kg BW) for 30 days and finally group T3B injected with D-gal at (150 mg/kg BW (i/p)) for 90 days. Blood samples were collected from the anesthetized rats to estimate the levels of blood urea nitrogen, cystatin C, protein carbonyl, catalase, tumor necrosis factor-alfa (TNF-α) and interleukin-1β (IL-1β) concentrations in serum. Furthermore, kidney tissue specimens have been collected for a histopathological investigation and to measure the expression of the kidney IL-10 gene. Results: The findings indicated a notable decrease in catalase, along with a significant elevation in blood urea nitrogen, cystatin C, albumin, protein carbonyl, TNF-α, and IL-1β levels in the T3B group. In addition, omega-3 displayed enhanced kidney barrier integrity; additionally, and T2 group showed reduced kidney inflammatory lesions and improved IL-10 gene expression. The findings showed that giving omega-3 to the T2 and T3A groups significantly enhanced kidney functions, inflammatory criteria, kidney barrier integrity, oxidative stress-related parameters, IL-10 gene expression, and histopathological abnormalities. Conclusions: Omega-3 exhibits preventive action against kidney dysfunctions induced by D-galactose in treated rats, as evidenced by modulation of kidney function tests and histopathological alteration through its antioxidant and anti-inflammatory capacities
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