Antibiotic susceptibility and molecular identification of Pseudomonas aeruginosa isolated from dog and cat wound samples
DOI:
https://doi.org/10.30539/fw39ae05Keywords:
Pseudomonas aeruginosa, antimicrobial resistance, companion animals, mexA and mexB genes, biofilm-producing isolatesAbstract
Background: Pseudomonas aeruginosa is an opportunistic pathogen frequently associated with wound infection in both humans and animals. Its intrinsic and acquired resistance to antibiotics poses a challenge in veterinary medicine. The emergence of multidrug-resistant strains in both pet and stray dogs and cats raises concerns regarding public health and treatment efficacy. This study aimed to compare antimicrobial resistance against P. aeruginosa isolated from wounds of pet and stray dogs and cats. Methodology: The VITEK 2 technique was used to identify these isolates, which were subsequently validated through molecular methods. Moreover, all isolates were tested for their antibiotic susceptibility using the disk diffusion method and their ability to form biofilm by the tube method. Results: P. aeruginosa was isolated from 3 of 42 cats and 2 of 17 dogs. All isolates exhibited the presence of the mexA and mexB genes, indicating the presence of specific antibiotic resistance mechanisms. Bacterial susceptibility to antibiotics revealed that the cat’s isolates showed some degree of sensitivity, ranging from 66% to 100%, to all tested antibiotics except ciprofloxacin, which showed 33% sensitivity. In dogs, the isolates showed 100% sensitivity to all listed antibiotics except ticarcillin-clavulanic acid, which showed intermediate resistance. Conclusions: The overall results of this study highlighted the clinical significance of P. aeruginosa in veterinary settings and illustrated the varying patterns of antibiotic susceptibility in animal hosts that were less exposed to antibiotics in comparison to human populations.
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